Mohana Marimuthu, Sanghyo Kim
Pump-less microfluidic perfusion holds O₂ within 5% of saturation across a 7-day mammalian-cell culture, demonstrating that gas-exchange membranes can replace active flow control for low-shear bioreactor operation.
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Open in lab →This research aims a continuous and uni- and the uniform oxygen/nutrient distribution continu- form oxygen tensions and oxygen gradients supply in ously supplied for dermal fibroblast cell culture. This microfluidic cell culture chip based micro-bioreactor could be a potential and effective model to be incor- without any external pumps by modifying the existing porated into tissue regeneration studies, drug screen- siphon based perfusion strategy using conventional ing model, and in cancer tissue model studies for un- tools to control constant hydrostatic pressure for con- derstanding angiogenesis, where oxygen tension and stant fluid flow rate. In this study, the microfluidic perfusion cultures play important roles. based micro-bioreactor is fabricated using a polydi- methylsiloxane (PDMS) replication process. The mi-